| ROLE | ACTOR |
| Zoosters | |
| Alex | Leo Gabriele |
| Marty | Katie Smolka |
| Gloria | Ana Maria Briscoe |
| Melman | Dyron Lyle |
| Zoo | |
| Zookeeper Zelda | Nora McCann |
| Zookeeper Zeke | James Murphy |
| Zookeeper Zoe | Abbey Diffendall |
| Zookeeper Zabrina | Campbell Diffendall |
| Zoo Guests | Maddie Baummer
Gina Brennan Clare Duhig Caroline McDermond Lidia McFaul Lilly Moreau Weston Osenberg Riley Thurman Bridget Tucker Sidonie Evans |
| Lionesses | Savannah Allen
Lauren Lee Christina Ndje Eliana Pohlhaus Kiran Turner Krassi Trainor |
| Mason | James Lancaster |
| Penguins | |
| Skipper | Chris DiMaggio |
| Kowalski | Thomas McFaul |
| Rico | Connor Counselman |
| Private | Caroline Scharpf |
| Penguin Squad | Mekhi Booker
Ella Gessner JP Gaarder Molly Waldron |
| Ship’s Captain | James Murphy |
| New Yorkers | |
| Candy Hammernose | Bridget Tucker |
| Camera Woman | Caroline McDermond |
| Police Officer #1 | James Murphey |
| Police Officer #2 | Lilly Moreau |
| Animal Control Officer | Maddie Baummer
Weston Osenberg |
| Newspaper Man | Gina Brennan |
| Old Lady | Clare Duhig |
| Passerby | Riley Thurman |
| Lemurs | |
| King Julien | Weston Osenburg |
| Maurice | Lauren Lee |
| Mort | Campbell Diffendall |
| Lynn | Nora McCann |
| Lew | Lidia McFaul |
| Lee | Krassi Trainor |
| Lars | Eliana Pohlhaus |
| Lemurs | Savannah Allen
Maddie Baummer Gina Brennan Abbey Diffendall Clare Duhig Ella Gessner Caroline McDermond Lilly Moreau Christina Ndje Riley Thurman Bridget Tucker Kiran Turner Molly Waldron Sidonie Evans |
| Ensemble | |
| Foosa Leader | James Lancaster |
| Foosa | Mekhi Booker
Connor Counselman JP Gaarder Thomas McFaul James Murphy |
| Servers | Maddie Baummer
Gina Brennan Clare Duhig Caroline McDermond Lidia McFaul Lilly Moreau James Murphy Christina Ndje Riley Thurman |
| Steaks | Savannah Allen
Abbey Diffendall Campbell Diffendall Lauren Lee Nora McCann Eliana Pohlhaus Kiran Turner Krassi Trainor |